Wavelength-multiplexing high-spectral resolution imaging based on Fourier ptychographic microscopy

Abstract

A wide field of view (FOV), high spectral resolution, and high spatial resolution are the targets of optical microscopic imaging. Fourier ptychographic microscopy (FPM) imaging can solve the contradiction between a wide FOV and spatial resolution. However, the spectral resolution of the samples is not accessible to typical LED-based FPM. Here, we report a wavelength-multiplexing high-spectral resolution reconstruction strategy based on the FPM of the halogen lamp. In contrast to the typical FPM setting, we can obtain spectral information of arbitrary wavelengths and greatly improve the sampling efficiency. We optimize and combine the wavelength-multiplexed decoupled algorithm with the high-spectral resolution reconstruction algorithm. We show that, by using our strategy, we can better reconstruct the image with a spectral resolution of 5 nm and maintain wide FOV and high spatial resolution. We believe the reported strategy may provide ideas for FPM-based spectral microimaging.

KEYWORDS:

• Fourier ptychographic microscopy
• high spatial resolution
• high spectral resolution
• wide FOV

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

Data underlying the results presented in this paper are available from the corresponding author on reasonable request.

Additional information

Funding

This work is supported by Zhejiang Provincial Natural Science Foundation of Zhejiang Province China under grant number LY22F050002. This work is also supported by the Graduate Scientific Research Foundation of Hangzhou Dianzi University under grant number CXJJ2023056.